Influence of temperature on adenovirus-mediated gene transfer
نویسندگان
چکیده
منابع مشابه
Adenovirus-mediated Gene Transfer to Liver Grafts
Background. Adenoviral gene therapy in liver transplantation has many potential applications, but current vector delivery methods to grafts lack efficiency and require high titers. In this study, we attempted to improve gene delivery efficacy using three different delivery methods to liver grafts with adenoviral vector encoding the LacZ marker gene (AdLacZ). Methods. AdLacZ was delivered to col...
متن کاملCanine adenovirus vectors: an alternative for adenovirus-mediated gene transfer.
Preclinical studies have shown that gene transfer following readministration of viral vectors is often inefficient due to the presence of neutralizing antibodies. Vectors derived from ubiquitous human adenoviruses may have limited clinical use because preexisting humoral and cellular immunity is found in 90% of the population. Furthermore, risks associated with the use of human adenovirus vecto...
متن کاملProapoptotic and antitumor activities of adenovirus-mediated p202 gene transfer.
PURPOSE AND EXPERIMENTAL DESIGN p202, a mouse IFN-inducible protein, is a member of the 200-amino acid repeat family. Enforced p202 expression in stable cancer cell lines resulted in growth inhibition in vitro and tumor suppression in vivo. However, to study the immediate effect of p202 and test the potential efficacy of p202 treatment, an efficient gene delivery system for p202 is required. Fo...
متن کاملCharacterization of adenovirus-mediated gene transfer in rabbit flexor tendons.
PURPOSE Adenoviral vector-based gene therapy is a promising technique for the delivery of growth factors to tendons. The objective of this study was to determine whether rabbit flexor tendons could be transduced effectively by adenoviral vectors and whether the introduction of adenoviral vectors would cause a notable local inflammatory response. METHODS Recombinant adenoviruses expressing gre...
متن کاملDecreased efficiency of adenovirus-mediated gene transfer in aging cardiomyocytes.
BACKGROUND Aging is an independent risk factor for the development of cardiovascular disease. Clinical application of myocardial gene transfer may be best suited in the elderly. In vivo gene transfer by adenovirus is less efficient in aging myocardium. METHODS AND RESULTS When infected with adenovirus containing beta-galactosidase (beta-gal) and green fluorescent protein (GFP) driven by cytom...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Cardio-Thoracic Surgery
سال: 1998
ISSN: 1010-7940
DOI: 10.1016/s1010-7940(98)00064-5